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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
26/07/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
HIRIGOYEN, D.; BRUZZONI, H.; AZAMBUJA, C.; STOLL, M. |
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Afiliación : |
D. HIRIGOYEN, División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.; H. BRUZZONI GIOVANELLI, División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.; CARLOS AZAMBUJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIO STOLL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Amplificación de ADN in vitro (PCR): instrumentación y metodología básica. |
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Complemento del título : |
Sección: Revisión. |
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Fecha de publicación : |
1992 |
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Fuente / Imprenta : |
Veterinaria, 1992, vol. 28, no. 115, p. 13-19. --- OPEN ACCESS. |
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Idioma : |
Español |
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Notas : |
Article history: Publicado 1 Marzo 1992. |
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Contenido : |
RESUMEN.- Desde la corta existencia de la biología molecular como ciencia varios aportes han impulsado la vertiginosa revolución tecnológica a la que hoy asistimos. La amplificación de ADN in vitro (PCR) es una poderosa ténica que permite identificar nuevos genes, así como cuantificar y caracterizar secuencias nucleotídicas. En este trabajo se presenta una breve descripción del revolucionario método (PCR), capaz de estimular la investigación científica en todos os órdenes y modificar el área diagnóstica en un futuro próximo. Se plantean brevemente algunos de los recientes desarrollos en su instrumentación y metodología. -------------------------------------- SUMMARY.- During the short time in which molecular biology exits as a science, several contributions have been made to improve the technical revolution we are attending nowadays. The DNA Polimerase Chain Reaction (PCR) is a technic that allows to identify new genes, to quantify and characterize nucleotid secuences. This paper makes a brief description of a revolutionary method that may improve scientific research and may modify diagnostic areas in a close future. Some of the recent developments, its instrumentation and methodology are establised here. |
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Palabras claves : |
Amplificación de ADN; Biochemical techniques; DNA amplification; Polymerase chain reaction (PCR); Reacción en cadena de la Polimerasa; Técnicas bioquímicas. |
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Thesagro : |
GENES. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17314/1/VM-1992-115-13-19.pdf
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Marc : |
LEADER 02043naa a2200253 a 4500
001 1004007
005 2023-07-26
008 1992 bl uuuu u00u1 u #d
100 1 $aHIRIGOYEN, D.
245 $aAmplificación de ADN in vitro (PCR)$binstrumentación y metodología básica.$h[electronic resource]
260 $c1992
500 $aArticle history: Publicado 1 Marzo 1992.
520 $aRESUMEN.- Desde la corta existencia de la biología molecular como ciencia varios aportes han impulsado la vertiginosa revolución tecnológica a la que hoy asistimos. La amplificación de ADN in vitro (PCR) es una poderosa ténica que permite identificar nuevos genes, así como cuantificar y caracterizar secuencias nucleotídicas. En este trabajo se presenta una breve descripción del revolucionario método (PCR), capaz de estimular la investigación científica en todos os órdenes y modificar el área diagnóstica en un futuro próximo. Se plantean brevemente algunos de los recientes desarrollos en su instrumentación y metodología. -------------------------------------- SUMMARY.- During the short time in which molecular biology exits as a science, several contributions have been made to improve the technical revolution we are attending nowadays. The DNA Polimerase Chain Reaction (PCR) is a technic that allows to identify new genes, to quantify and characterize nucleotid secuences. This paper makes a brief description of a revolutionary method that may improve scientific research and may modify diagnostic areas in a close future. Some of the recent developments, its instrumentation and methodology are establised here.
650 $aGENES
653 $aAmplificación de ADN
653 $aBiochemical techniques
653 $aDNA amplification
653 $aPolymerase chain reaction (PCR)
653 $aReacción en cadena de la Polimerasa
653 $aTécnicas bioquímicas
700 1 $aBRUZZONI, H.
700 1 $aAZAMBUJA, C.
700 1 $aSTOLL, M.
773 $tVeterinaria, 1992, vol. 28, no. 115, p. 13-19. --- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
PÉREZ, C.A.; WINGFIELD, M.J.; SLIPPERS, B.; ALTIER, N.; BLANCHETTE, R.A. |
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Afiliación : |
C.A. PÉREZ, Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA; Departamento de Protección Vegetal, EEMAC, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; M.J. WINGFIELD, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; B. SLIPPERS, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa; NORA ADRIANA ALTIER MANZINI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; R.A. BLANCHETTE, Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA. |
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Título : |
Endophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Fungal Diversity, 2010, v. 41 no.1, p. 53-69. |
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ISSN : |
1560-2745 |
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DOI : |
10.1007/s13225-009-0014-8 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 August 2009 / Accepted: 7 October 2009 / Published online: 15 January 2010. |
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Contenido : |
ABSTRACT.
Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from native trees and identified as L. pseudotheobromae, N. eucalyptorum and the N. parvum-N.ribis complex are pathogenic to E. grandis. Interestingly, Lasiodiplodia pseudotheobromae has not previously been found on Eucalyptus in Uruguay and represents a potential threat to this host.
© Kevin D. Hyde 2010. MenosABSTRACT.
Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from ... Presentar Todo |
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Thesagro : |
AGENTES PATÓGENOS; BOTRYOSPHAERIA; ENFERMEDADES FORESTALES; PATÓGENOS FORESTALES. |
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Asunto categoría : |
K10 Producción forestal |
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Marc : |
LEADER 02678naa a2200253 a 4500
001 1051308
005 2019-10-15
008 2010 bl uuuu u00u1 u #d
022 $a1560-2745
024 7 $a10.1007/s13225-009-0014-8$2DOI
100 1 $aPÉREZ, C.A.
245 $aEndophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay.$h[electronic resource]
260 $c2010
500 $aArticle history: Received: 19 August 2009 / Accepted: 7 October 2009 / Published online: 15 January 2010.
520 $aABSTRACT. Species of the Botryosphaeriaceae are important pathogens causing cankers and die-back on many woody plants. In Uruguay, Neofusicoccum eucalyptorum, N. ribis and B. dothidea have previously been associated with stem cankers on plantation-grown Eucalyptus globulus. However, very little is known regarding the occurrence and species diversity of Botryosphaeriaceae in native Myrtaceae forests or what their relationship is to those species infecting Eucalyptus in plantations. The objectives of this study were to identify the Botryosphaeriaceae species present as endophytes or associated with cankers in both introduced and native tree hosts in Uruguay, and to test the pathogenicity of selected isolates obtained from native trees on Eucalyptus. Symptomatic and asymptomatic material was collected countrywide from Eucalyptus plantations and native Myrtaceae trees. Single spore cultures were identified based on conidial morphology and comparisons of DNA sequences of the ITS and EF1-α regions. Six Botryosphaeriaceae species were identified. Botryosphaeria dothidea, N. eucalyptorum and specimens residing in the N. parvum-N. ribis complex were isolated from both introduced Eucalyptus and native Myrtaceae trees, whereas Lasiodiplodia pseudotheobromae was found only on Myrcianthes pungens. Diplodia pseudoseriata sp. nov. and Spencermartinsia uruguayensis sp. nov. are novel species found only on native myrtaceous hosts. Pathogenicity tests showed that isolates obtained from native trees and identified as L. pseudotheobromae, N. eucalyptorum and the N. parvum-N.ribis complex are pathogenic to E. grandis. Interestingly, Lasiodiplodia pseudotheobromae has not previously been found on Eucalyptus in Uruguay and represents a potential threat to this host. © Kevin D. Hyde 2010.
650 $aAGENTES PATÓGENOS
650 $aBOTRYOSPHAERIA
650 $aENFERMEDADES FORESTALES
650 $aPATÓGENOS FORESTALES
700 1 $aWINGFIELD, M.J.
700 1 $aSLIPPERS, B.
700 1 $aALTIER, N.
700 1 $aBLANCHETTE, R.A.
773 $tFungal Diversity, 2010$gv. 41 no.1, p. 53-69.
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